Fig. 5.

Antibody induces the differentiation of dendritic cells from human bone marrow CD34⁺ cells. CD34⁺ cells were cultured on 24-well tissue-culture plates with 12-1/12-2 antibodies or PBS for 10 d. (A) (Upper) The common type of CD34⁺ and dendritic cell-like morphology by bright-field microscopy. (Lower) Dendritic cell-like morphology by SEM and TEM. (B) PBS- or antibody-treated CD34⁺ cells were stained with phycoerythrin (PE)-conjugated anti-human CD11c antibody or DAPI and analyzed by confocal microscopy.