Fig. 6.

RGD motif of the antibody is critical for the activity of the antibody. (A) CD34⁺ cells were treated with 12-1/12-2 antibodies (containing RGD) or a mutant form of 12-1/12-2 antibodies (containing RGE), an inactive control antibody, or PBS for 10 d. These cells were stained with a PE-conjugated anti-human CD11c antibody and FITC-conjugated anti-human F4/80 antibody and analyzed by flow cytometry. CD11c and F4/80 are specific markers of dendritic cells and macrophages, respectively. (B) CD34⁺ cells were incubated with PBS, 12-1/12-2 antibodies, or the mutant form of 12-1/12-2 antibodies for 10 min at 37 °C and analyzed by Western blotting with anti–p-AKT, AKT, p-ERK, and ERK antibodies.