Fig. 3.

Protrudin plays a role in forming the tubular ER network. (A) Two different siRNAs against human protrudin were transfected into HeLa cells for 72 h, and cell lysates were immunoblotted. An asterisk (*) indicates a cross-reacting band, and an arrow identifies protrudin. Migrations of molecular-mass standards are to the right. (B) siRNA-transfected HeLa cells were immunostained for CLIMP-63 (green) and REEP5 (red). Insets are enlarged to the right of each image. (Scale bars: 10 μm.) Line-scan plots are shown in Fig. S6A. (C) Numbers of cells with expanded CLIMP-63 signal area were quantified in control and protrudin-depleted conditions (n = 3; >200 cells per experiment). Means ± SD are shown. Paired Student t test: **P < 0.01; ***P < 0.001.