Fig. 2.
Germ-line gene of Pacific hagfish 3rdVLR and its comparison with previously described agnathan VLR genes. (A) Germ-line gene sequence of the hagfish 3rdVLR (GenBank accession no. KF314110). The gene (719 nucleotides in length) possesses two coding regions separated by an intervening sequence. Arrowed lines indicate the forward and reverse primer regions used for 3′-RACE and cDNA cloning. (B) Schematic depiction of the hagfish and lamprey VLR genes (not drawn to scale). Note the distinctive structure of the hagfish 3rdVLR germ-line gene.