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. 2013 Aug 26;110(37):15025–15030. doi: 10.1073/pnas.1303193110

Fig. 2.

Fig. 2.

The ERK pathway is antiviral in mosquito cells. (A) A. aegypti Aag2 cells treated with the indicated dsRNAs were challenged with VSV (MOI = 0.01) or SINV (MOI = 0.5) and monitored by microscopy (virus, green; nuclei, blue). (B) Quantification of A with mean ± SD of three independent experiments; *P < 0.05. (C) Immunoblot analysis of Aag2 cells treated as in A. (D) qRT-PCR analysis of viral RNA normalized to Rp49 and shown relative to control cells treated as in A. Mean ± SD of three independent experiments; *P < 0.05. (E) Immunoblot analysis of Aag2 cells treated with insulin (3.4 µM) or U0126 (10 µM) for 15 min. (F) Aag2 cells treated with PBS or U0126 (10 µM), infected with the indicated virus, and monitored by microscopy. (G) Quantification of images in F with mean ± SD of three independent experiments; *P < 0.05. (H) Immunoblot analysis of Aag2 cells infected with SINV for 60 min.