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. 2013 Jun 4;2(3):227–242. doi: 10.1016/j.molmet.2013.05.006

Figure 4.

Figure 4

Adipocyte ERα regulates adipocyte size and gene function. (A) Representative photomicrographs of H&E staining of gonadal WAT from 18-week-old male and female mice. (B) Analysis of adipocyte cell size in both male and female of gonadal adipose tissue from 18-week-old mice (n=10/genotype). (C) Messenger RNA levels of F4/80 were quantified using qPCR whole adipose tissue from 18-week-old chow-fed females and males (n=10/genotype). (D) Messenger RNA levels of inflammatory genes were quantified using qPCR in collagenase-isolated adipocytes from 18-week-old chow-fed females and males (n=10/genotype). (E) Messenger RNA levels of fibrosis genes were quantified using qPCR in collagenase-isolated adipocytes from 18-week-old chow-fed females and males (n=10/genotype). Data are presented as mean±SEM, *P<0.05 between WT and AdipoERα mice, and ♯P<0.05 between male and female mice of like genotype.