Supplementary Fig. 1.

Using an immunoprecipitation of adipose tissue from both WT and FGF21KO mice treated with either vehicle (−) or rosiglitazone (+) we were unable to detect either basal or ligand stimulated sumoylation of PPARγ. Detection of PPARγ was hindered in in vivo samples by detection of endogenous mouse antibodies at the correct molecular weight for PPARγ.