Figure 2.

PER2 is required for normal circadian expression of the liver clock and glucose output genes. (A) Real-time qPCR analysis of the expression of clock genes in livers from WT and Per2^Brdm1 mice collected at the indicated zeitgeber times (ZT). (B) Real-time qPCR analysis of the samples described above for key liver glucose output genes. Pyruvate tolerance tests were performed starting at either ZT2 (C) or ZT14 (D) on 16 h-fasted WT and Per2^Brdm1 mice. Data are expressed as means, standard errors are indicated, and P values were calculated using the Student t-test. Phosphoenolpyruvate carboxykinase (PEPCK); glucose 6-phosphate transporter (G6PT); Glucose transporter 2 (GLUT2).