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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Biochim Biophys Acta. 2013 Apr 10;1830(8):4218–4228. doi: 10.1016/j.bbagen.2013.04.005

Figure 6. Effect of covalent irreversible inhibitors on RgpB-PD complex formation.

Figure 6

RgpB (10 µM) was pre-incubated with TLCK, Z-FR-FMK, H-D-FFR-CMK, and YPR-CMK (each at 100 µM) in reducing buffer before equimolar amount of PDRgpB was added. After 15–20 min incubation, samples were subjected to (A) size-exclusion chromatography on Superdex™ 200 10/300 GL; (B) native PAGE followed by (C) Western blot analysis using anti-RgpB antibody.