Figure 7. The effect of the substrate structure on the mode of inhibition of RgpB by PDRgpB.
The mode of inhibition was determined graphically using the Cornish-Bowden plot of S/V versus I, where [S] substrate concentration; Vo, initial rate (velocity) of the reaction; I, inhibitor concentration. Substrates used: (A) Z-FR-AMC, (B) Z-GPR-AMC and (C) Z-AGPR-AMC. Non-competitive inhibition lines intersect on the abscissa (S/v = 0) at I = -Ki); competitive inhibition, lines are parallel.