Validation of methodology on simulated images of triblock copolymers, and the effects of window size. To validate the methodology we show results for the sliding window method for simulated ensembles of contours (M = 500, no image noise) generated with known heterogeneous elasticity (in gray), with Lp(0) = Lp(L) = 0.5 mm, and Lp(L/2) = 2.5 mm. The width of the stiffer middle sub-segment size was varied from ℓ = L/3 to L/10 and persistence lengths determined using four window widths (L/4 in black, L/5 in red, L/6 in green, and L/8 in blue).