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. 2013 Sep 2;2013:732126. doi: 10.1155/2013/732126

Figure 3.

Figure 3

Effects of the water extract of VN on adipogenesis and transcription factors in 3T3-L1 cells. An MTS assay (a), Oil Red O staining assay (b), real-time RT-PCR assay (c), and Western blot assay (d) were performed to measure the effects of VN in 3T3-L1 cells. 3T3-L1 preadipocytes were induced to differentiate with 10% FBS/DMEM medium containing insulin, DEX, IBMX, and 0, 0.01, 0.1, or 1 mg/mL of VN extract for 2 days, and then the culture media were replaced with 10% FBS/DMEM medium containing insulin for the following 2 days. The control was treated with DW instead of extracts. EGCG was used as a positive control. Assays were performed in duplicates for each concentration, and experiments were repeated at least three times. Data represented are the relative expression. All values are mean ± SD. # P < 0.05, versus undifferentiated control cells; *P < 0.05 and **P < 0.005, versus differentiated control cells; DW, distilled water; VN, Veratrum nigrum; EGCG, epigallocatechin gallate.