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. Author manuscript; available in PMC: 2014 Mar 5.
Published in final edited form as: Nature. 2013 Aug 21;501(7465):52–57. doi: 10.1038/nature12479

Figure 1. S. aureus infection induces pain hypersensitivity paralleling bacterial load but not immune activation.

Figure 1

(a) S. aureus infection induces mechanical hypersensitivity (p=0.0021, n= 10/group), heat hypersensitivity (p<0.0001, n=10/group), acetone cold response (p<0.0001, n=20/group), and tissue swelling (p<0.0001, n=10/group). *p<0.05, ***p<0.001. (b) Left, flow cytometry shows myeloid (CD11b+CD45+) but not lymphoid (CD11b−CD45+) immune expansion in infected tissues. Right, Quantification of infected tissue neutrophils (CD11b+Ly6G+), Ly6chi monocytes (CD11b+Ly6G−Ly6Chi), and Ly6Clo monocytes/macrophages (CD11b+Ly6G−Ly6Clo). n=3/time-point. (c) TNF-α, IL-1β levels in infected tissues. n=4/time-point. (d) Bacterial load recovery. n=4/time-point. (e) GFP-S. aureus are in proximity with Nav1.8-Cre/TdTomato+ dermal nerve fibers, 3 hours post-infection. Scalebar,100 μm. Error bars, mean±s.e.m.