Figure 4.

Contractility and Ca²⁺ handling of isolated cardiomyocytes 22h after CASP. A: Representative tracings of changes in sarcomere length (SL) and intracellular Ca²⁺ transients ([Ca²⁺]_i) of isolated cardiomyocytes paced at 2 Hz. B–E: Effects of CASP on the contractile function and Ca²⁺ handling of isolated cardiomyocytes paced at 2 Hz. Data were summarized for peak percent SL shortening (B), peak amplitude of [Ca²⁺]_i (Δ[Ca²⁺]_i) (C), the time constant for SL relengthening (D) and the time constant for [Ca²⁺]_i decay (E). 20–25 cells from 3 – 5 mice were analyzed for each group. *P<0.05 vs cardiomyocytes from control mice of the same genotype, #P<0.05 vs cardiomyocytes from WT mice after CASP. F: Effects of CASP on relationship of peak systolic [Ca²⁺] and %SL shortening.