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Immunoblot analysis. Protein expression of SR Ca2+-ATPase2a (A), fractions of phospholamban phosphorylated at Ser16 (PLN Ser16 (B)) or Thr17 (PLN Thr17 (C)) in cardiac tissue extracts from WT and NOS3KO mice 22 h after CASP or sham operation are shown with loading control (β-tubulin). Data were normalized to WT sham groups. Representative blots from 2 – 4 mice for each group are shown. N = 6 – 8 for each group.
