Figure 4.

Effect of ATM on AmB-tolerant cells in biofilms of C. albicans CA-IF100 (a) and clinical isolates F17 and G6 (b). C. albicans biofilms were treated without (/) or with 1 μM AmB in the absence (−) or presence of 10 mM ATM. Afterwards, biofilms were washed with PBS and survival of Candida cells was determined by plating the biofilm cells on YPD plates. The AmB-tolerant fraction in presence or absence of 10 mM DDC was determined relative to DMSO treatment. Data represent the mean and SEM for one representative experiment out of two, each consisting of triplicate measurements. (a) CA-IF100 and (b) clinical isolates F17 (white bars) and G6 (black bars). **P < 0.01; ***P < 0.001.