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. Author manuscript; available in PMC: 2013 Sep 16.
Published in final edited form as: Vet Immunol Immunopathol. 2008 Oct 17;128(0):30–36. doi: 10.1016/j.vetimm.2008.10.292

Fig. 2.

Fig. 2

SDS-PAGE and immunoblot analysis of cell lysates from SEC1-stimulated bovine PBMCs. Bovine PBMCs were exposed to SEC1 (5 ng/ml) for up to 8 d. PBMCs were harvested at 2, 4, 6, or 8 d of culture. Cell lysates were resolved by SDS-PAGE and stained with Coomassie Blue (A) or transferred to membranes and probed with FOX20A mAb (B). panel A, arrows indicate location of bands corresponding to the bands detected in immunoblots (panel B). Lane M, prestained markers (Invitrogen); Lane1, 2, 3, and 4 represent lysates from days 2, 4, 6, and 8, respectively. To ensure equal sample loading, membranes were stripped and reprobed with an anti-beta actin mAb (Beta actin panel).