Skip to main content
. Author manuscript; available in PMC: 2013 Sep 16.
Published in final edited form as: Vet Immunol Immunopathol. 2008 Oct 17;128(0):30–36. doi: 10.1016/j.vetimm.2008.10.292

Fig. 5.

Fig. 5

Flow cytometric analysis of FOXP3 expression. Bovine PBMCs stimulated with SEC1 up to 8 d were analyzed by triple labeling for CD4, CD25, and FOXP3. Dot plots showed expression of CD4 and/or CD25 following exposure to SEC1. CD4+ CD25− (upper left corner) or CD4+ CD25+ T cells (upper right corner) were gated to monitor the expression of FOXP3 in these subpopulations. The histogram shows expression of FOXP3 gated in CD4+ CD25− or CD4+ CD25+ T cells (Dotted lines: isotype control; solid lines: results using Fox20A mAb). Data shown are from a single representative experiment which was conducted three times.