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. 2012 May 30;303(4):F503–F514. doi: 10.1152/ajprenal.00170.2012

Fig. 2.

Fig. 2.

HIV-induced tubular cell VDR downregulation is mediated through reactive oxygen species (ROS). A: EV/HCs, HIV/HCs, HIV/HCs + catalase (CAT, 200 U) were incubated in SFM for 24 h. Subsequently, cellular lysates were electrophoresed and probed for VDR and actin (n = 3). The lane on top shows VDR expression by control and experimental cells. The lane on bottom shows actin content of the cells under similar conditions. B: total RNA was extracted from the cells treated under conditions mentioned in A (C/HCs, EV/HCs, HIV/HCs, and HIV/HCs + catalase). VDR expression was measured by real-time PCR studies (n = 3). *P < 0.01 compared with C/HC and EV/HC. **P < 0.05 compared with HIV/HC. C: HCs were incubated in SFM containing variable concentrations of H2O2 (0, 25, 50, 100, 250, and 500 μM) for 12 h. Subsequently, cellular lysates were electrophoresed and probed for VDR and actin. The lane on top shows VDR expression by control and experimental cells. The lane on bottom shows actin content of these cells.