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. 2012 Jun 27;303(6):C635–C644. doi: 10.1152/ajpcell.00147.2012

Fig. 6.

Fig. 6.

Analysis of cononical transient receptor potential (TRPC) channel expression at the mRNA and protein levels. A: PCR analysis of guinea-pig B, AC, and AM cDNAs for TRPC1–TRPC7 and β-actin. PCR products (TRPC1, 367 bp; TRPC2, 317 bp; TRPC3, 322 bp; TRPC4, 498 bp; TRPC5, 471 bp; TRPC6, 472 bp; TRPC7, 475 bp; β-actin, 198 bp) for each of the 7 TRPC subtypes and for β-actin were detected in brain cDNAs. Those for all the TRPC subtypes except TRPC2 were detected in AM cDNAs, whereas those for TRPCs 1, 4, 5, and 7 were recognized in AC cDNAs. B: immunoblotting of homogenates of guinea-pig AC, AM, and B for TRPCs 4, 5, and 7. C: detection of TRPC1 in guinea-pig B and AM. Homogenates of B and AM were subjected to immunoprecipitation (IP) with Santa Cruz-made anti-TRPC1 Ab (S) and rabbit IgG (C) and then to immunoblotting (IB) with Alomone-made anti-TRPC1 Ab (A). Arrowhead represents bands of heavy chain of IgG.