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. Author manuscript; available in PMC: 2013 Sep 16.
Published in final edited form as: J Immunol. 2010 Dec 22;186(3):1442–1449. doi: 10.4049/jimmunol.1003023

FIGURE 5.

FIGURE 5

Frequencies of cytokine-producing T cells in naive and transplanted mice. The frequencies of alloreactive anti-Kb T cells secreting proinflammatory type 1 cytokines IL-2 (A), IFN-γ (B), and type 2 “regulatory” cytokines IL-4 (C) and IL-10 (D) were measured by ELISPOT. Spleen cells from nontransplanted (naive, white bars) and mice recipient of a CBK heart transplant (10 d post-transplant, solid bars) were collected and stimulated in vitro with irradiated CBK Kb Tg splenocytes (MLR). The results are presented as cytokine-producing spots per million T cells ± SD. The results are representative of four experiments each including two to three mice tested individually.