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. Author manuscript; available in PMC: 2013 Sep 16.
Published in final edited form as: J Endocrinol. 2011 Mar 23;209(3):327–335. doi: 10.1530/JOE-10-0493

Fig. 2. Major steroids produced by AA cells with/without ACTH treatment.

Fig. 2

Primary human adrenal culture cells were isolated as described in Materials and Methods, and plated at a density of 300,000 cells/well in 24-well dishes. Cells were treated with/without ACTH (10 nM) for 48 h before harvest. The concentration of steroids in the medium was measured by LC-MS/MS and normalized to the amount of protein. The amount of four major steroids produced by AA cells, cortisol, corticosterone, 11-deoxycortisol and 11OHA are shown in the graphs with each line representing an individual, independent experiment.