Skip to main content
. Author manuscript; available in PMC: 2013 Sep 16.
Published in final edited form as: J Endocrinol. 2011 Mar 23;209(3):327–335. doi: 10.1530/JOE-10-0493

Fig. 3. Time-dependent stimulation of three major steroids produced in AA cells by ACTH treatment.

Fig. 3

Primary human adrenal culture cells were isolated as described in Materials and Methods, and plated at a density of 300,000 cells/well in 24-well dishes. Cells were treated with/without ACTH (10 nM) for indicated times. The concentration of steroids (cortisol, corticosterone and 11OHA) in the medium was measured by immunoassay and expressed as fold changes over basal level. Results represent means ± S.E.M of data from at least three experiments using cells isolated from different adrenal glands.