Figure 3. SKN-1 Is Activated by Starvation and Changes in Available Nutrients.
(A) GO-terms of genes identified as dysregulated by transcriptional analysis of the lax120 and lax188 mutants.
(B) SKN-1 activity sensor worms were grown on food or under starvation conditions and then imaged for GFP expression indicative of SKN-1 activation of the gst-4p::gfp reporter. (C) Quantification of GFP levels observed as described in (B).
(D–I) Indicated strains were fed the E. coli B strain OP50.
(D)–(F) or the E.coli K12 strain HT115 (G)–(I) and imaged for GFP expression as described in (B).