Figure 2.

Nrp1^cyto Mice Show Impaired Blood Flow Recovery in the Hindlimb Ischemia Model

(A and B) Blood flow measurements in the HLI model. (A) Doppler imaging demonstrated the level of perfusion in hindlimbs of WT and Nrp1^cyto mice before and after HLI surgery at the indicated time points. (B) Quantitative analysis of laser Doppler images 3 days after HLI surgery in WT (black bars) and in Nrp1^cyto mice (mean ± SEM, ^*p < 0.05).

(C–F) Micro-CT analysis of arterial diameter in the HLI model. Representative micro-CT images of (C) WT and (D) Nrp1^cyto mice 14 days after HLI surgery. Quantitative micro-CT analysis of arterial vasculature (E) above and (F) below the knee in WT (black bars) and Nrp1^cyto mice (white bars) 14 days after HLI surgery. The total number of <100 μm diameter vessels was significantly decreased in Nrp1^cyto mice relative to WT littermates in both the thigh and calf (mean ± SEM, ^*p < 0.05).

See also Figure S3.