Table 2.
Major primers used in this study
| Primer | DNA Sequence (5′-3′)a | Product size (bp) |
Target gene/ operon |
|---|---|---|---|
| Cj1376_F | TTTATCGCTATGGGCTTTGC | 1,917 | C. jejuni cee locus |
| Cj1376_R | TTGCAAAATGTTTTAAAAGAGCA | ||
| Cm_PacI_F | CCCTTAATTAATGCTCGGCGGTGTTCCTTT | 801 | Chloramphenicol resistance cassette |
| Cm_PacI_R | CCCTTAATTAAGCGCCCTTTAGTTCCTAAAG | ||
| TonB3F | TGGCAACACTTTACATAG | 1,343 | tonB3 with promoter |
| TonB3R | CATTGATAGTAGCAGGAG | ||
| rrsF | CTGGAACTCAACTGACGCTAAG | 1,900 | Ribosomal DNA |
| rrlR | CTCTTGCACATTGCAGTCCTAC | ||
| CfrB_XbaI_F | GCTCTAGATGGAGCCTATCAAGAGGCTTAG | 2,409 | cfrB with promoter |
| CfrB_MfeI_R | GCGCAATTGCCAAGTGCAAAGCCTACCAT | ||
| Erm_MfeI_F | GCGCAATTGAGCTTTGGCTAACACACACG | 1,140 | Erythromycin resistance cassette |
| Erm_MfeI_R | GCGCAATTGAATAGGTACACGAAAAACAAGT TAAGG |
a
Restriction sites are underlined in the primer sequence.