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. Author manuscript; available in PMC: 2013 Sep 16.
Published in final edited form as: Mol Immunol. 2012 May 10;52(1):9–18. doi: 10.1016/j.molimm.2012.04.004

Figure 5. Cyclophosphamide (CYP) accelerates changes that naturally occur between 10 weeks of age and TID onset.

Figure 5

Eight week old female NOD mice were treated with (n=10) or without (n=20) CYP and monitored for diabetes onset. One week after the last CYP injection when all treated mice were diabetic, PLN were isolated and the CDR3 spectratype for all 19 TCR Vβ families determined for individual mice. (A) Total perturbation index for Vβ families that expand naturally and after CYP treatment between 10 weeks of age and TID onset. (B) Total perturbation index for Vβ families that expand only after CYP treatment. The total perturbation index for each Vβ family was calculated for each mouse. The mean and SD is shown for each Vβ family, and each dot represents an individual mouse. (C) Global perturbation index for treated and untreated mice. The global perturbation index was calculated for each mouse and the mean ± SD is shown. Statistical analysis was performed using unpaired t test and statistical differences between the corresponding groups is shown as *** p<0.0001; ** p<0.001 or * p<0.01.