Fig. 1.

Immunoblots showing whole homogenate protein samples from human colonic tissue probed using the affinity-purified UT-B urea transporter antisera (BUTB-PAN) antibody either with or without 2-h preincubation in 1 μg/ml of specific immunizing peptide. BUTB-PAN detected signals at 30 and 35 kDa within human colon protein. Deglycosylation reduced the 35-kDa band down to 30 kDa, while having no further effect on the 30-kDa signal. These signals were absent when BUTB-PAN was preincubated in its immunizing peptide.