Fig. 2.

A: immunoblot comparing protein from colonic mucosa and colonic muscle probed with BUTB-PAN antibody. Colonic mucosa contained the vast majority of the 30- and 35-kDa signals, with only the 30-kDa band present in colonic muscle. The 35-kDa protein was predominantly located in the plasma membrane enriched protein samples, while the 30-kDa signal was strongest in the intracellular membrane-enriched protein. There was minimal degraded UT-B protein present at the dye front of the immunoblot. DF, dye front of protein samples; Wh, whole homogenate; 17, 17,000-g pellet containing plasma membrane-enriched protein; SN, 17,000-g supernatant containing intracellular membrane-enriched protein. B: summary of immunoblot densitometry results for UT-B signals within different human colonic mucosal samples. *P < 0.05, ***P < 0.001 vs. supernatant signal by ANOVA (n = 6).