A: comparison of UT-B protein abundance detected by BUTB-PAN in different sections of the human colon against the amount of protein loaded, as shown by ProtoBlue gel staining. Immunoblot suggests that abundance level of glycosylated 35-kDa UT-B protein varies in different sections of the human colon. As, ascending colon; Tr, transverse colon; De, descending colon; Si, sigmoid colon. B: deglycosylation immunoblots confirm differential abundance of the 35-kDa glycosylated UT-B protein between ascending and descending colon.