Fig. 6.

NLC parameters change as a function of time after induction. A: development of specific charge density (Q_sp) after induction in a representative tetracycline-inducible prestin cell line (16c) with time. Q_sp increase shows a sigmoidal pattern with time and stabilizes at 30+ h. The number of cells patched for each point ranged from 4 to 11. Error bars are ±SE. Data were fitted to a Hill equation [y = start + (end − start) × x∧n/(k∧n + x∧n)] with the following parameters: start = 1.23; end = 32.0; k = 18.3; n = 2.0. B: development of unitary charge valence (z) in a tetracycline-inducible cell line as a function of time after induction. z shows a rapid increase over several hours to reach its plateau at 6–8 h. z values did not change significantly, remaining stable after this time point. Fitting parameters were as follows: start = 0.58; end = 0.80; k = 3.0; n = 6.8. C: development of voltage dependence of peak NLC (V_h) as a function of time after induction. While there was considerable variation in V_h values, there was a clear trend in the development of V_h values toward more depolarizing voltages with time. Fitting parameters were as follows: start = −123; end = −69.4; k = 8.6; n = 0.74.