Fig. 8.
Prestin's lower monomer exerts a dominant-negative effect on the upper functional monomer. A: the relationship between Qsp and the densitometric ratio of the upper to lower prestin monomer expressed on the surface of the cell is linear (Qsp = −8.35 +11.12 × ratio; adjusted R2 = 0.93). Data were obtained from cells at variable time points after induction. Taken together, these data would suggest that the lower monomer exerts a dominant-negative effect on the function of the molecule. Also shown is the relationship between the upper monomer intensity and Qsp. There is an initial correlation between the two that decreases with higher Qsp values. B: correlation of z and Vh in the initial phase after induction where there is a rapid increase in z that correlates with a change in Vh.