Fig. 6.
Superoxide anion (O2·−) and lipid peroxidation are increased in SHR and Iso-rats. A: O2·− generation by myocardial slices from control (■), Iso (▴), and SHR (∘). Both Iso and SHR display a significant increase in O2·− production. The data fitted with a one-phase exponential association (GraphPad Prism 4.2) from SHR and Iso-rats were significantly higher than the control curve. For better comparison, the bar graph below depicts the mean values at 20 min, when the reaction was completely stabilized, showing a significant increment in O2·− generation in Iso and SHR with respect to control rats. AU, arbitrary units; CPM, counts/min. B: average data of thiobarbituric acid reactive substances (TBARS), indicating that SHR and Iso-rats present higher levels of lipid peroxidation than control rats. Values are means ± SE; n, no. of animals in each experimental group (in parentheses). *P < 0.05 vs. control.