Fig. 3.

Analysis of protein levels of VEGFR1, VEGFR2, and VEGF. A: temporal protein expression of VEGFR1, VEGFR2, and VEGF was determined by Western blotting, as detailed in materials and methods. Analyses included PFDs 1, 3, 7, and 14, which corresponded to hemorrhage formation, inflammatory response, initiation of callus development, and the presence of a mature callus, respectively, as well as IB. Antibody specificity was verified carefully using appropriate positive controls (antibody control). B: equal amounts of protein extracted from PFDs 1, 3, 7, and 14 and IB were probed against housekeeping proteins GAPDH and β-actin. The Western blot for GAPDH is shown at two exposure times (10 s and 1 min). C: equal amounts of protein extracted from bone, bone marrow, and muscle derived from the 8th rib were probed against GAPDH and β-actin.