Table 1.
Experimental design
| Day of Tissue Harvest | No. of Animals | Methods |
|---|---|---|
| Postfracture day | ||
| 1 | 8 | Histology and IHC |
| 5 | Protein extraction | |
| 5 | RNA extraction | |
| 3 | 8 | Histology and IHC |
| 5 | Protein extraction | |
| 5 | RNA extraction | |
| 5 | 8 | Histology and IHC |
| 7 | 8 | Histology and IHC |
| 5 | Protein extraction | |
| 5 | RNA extraction | |
| 14 | 8 | Histology and IHC |
| 5 | Protein extraction | |
| 4 | RNA extraction | |
| 21 | 8 | Histology and IHC |
| Intact bone | 4 | Histology and IHC |
| 5 | Protein extraction | |
| 5 | RNA extraction | |
| 1 | Protein extraction for musculoskeletal tissue analysis |
IHC, immunohistochemistry.