Figure 1.

One QTL affects both WUE and FT and is associated with functionally divergent FRI alleles. (a) The WUE–FT correlation observed in nature is present within our recombinant mapping population; bivariate breeding values for each TK RIL (hollow points) and the linear model (solid line) are plotted. (b) Standardized LOD profile scores for both WUE (dashed blue) and FT (solid red) colocalize at 4 cM on chromosome 4. (c) WUE and FT of each RIL are split by genotype and plotted with means ± s.e. of the mean. (d) The only significant expression difference within 100 kb of the QTL point estimate (chromosome 4287.06 kb) is at FRI (labelled). Horizontal line: FDR = 0.05. (e) Precise co-localization of the main QTL for WUE and FT is shown: standardized, summed LOD profiles are plotted for each pairwise locus combination across the first 20 cM of Ch4. Grey bars on the axes indicate the point where the maximum score is achieved.