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Morphological assessment of the large intestine. Large intestinal tissue was collected male mice and fixed in 4% paraformaldehyde at 4°C overnight, dehydrated, and embedded in paraffin. Sections were stained with hematoxylin and eosin (H&E) observed under microscope (×100). +/+: Wild-type mice; −/−: NHE8−/− mice. A: colonic section from 2-wk-old mice. B: colonic section from 8-wk-old mice.
