Table 1.
Primers used to identify insertion position in NHE8 gene
| Name | Direction | Location | Position, bp | Sequence |
|---|---|---|---|---|
| In3F1 | Forward | Intron 3 | 119–139 | GTTTGGTTGATTGTGAACGA |
| In3F2 | Forward | Intron 3 | 983–1,002 | TCCTAGCTGAGTGGCGATG |
| TrapR3 | Reverse | pGT0Lxf | 78–103 | GGTTGGAGTGTGTGTTGTAGGACACG |
| TrapR2 | Reverse | pGT0Lxf | 1,616–1,647 | GTAAAACGACGGGATCCTCTAGAGTCCAGATC |
| A | Forward | Exon 2 | 80–102 | ACCCTTCACACCACCTTGGGTGT |
| B | Reverse | Exon 2/3 | 216–240 | AGGATGATGCAGATGGCTAGGACGA |
| C | Reverse | Exon 4/5 | 353–371 | CTTCCTCCTTCCAATTTGC |
| D | Forward | Exon 13 | 1,331–1,350 | CTGGAGCCCATGGAGAAGCG |
| E | Reverse | Exon 14 | 1,735–1,753 | CTCCTGCTCATCATCCTCA |