Fig. 1.

Schematic illustrations of ghrelin-green fluorescent protein (GFP) transgene construct. A: construct of pInsulator-GFP. Two copies of β-globin insulator were inserted at 5′ and 3′ sides of the coding sequence for enhanced GFP (EGFP) and SV40 poly(A) sequences. B: final construct of ghrelin-GFP transgene, Pghrelin-GFP. Mouse ghrelin gene sequence −5543 to +18 bp upstream of the translational start site was amplified by PCR and then inserted in frame with the GFP gene sequences between restriction enzyme KpnI and SmaI sites.