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. 2000 Jan 15;105(2):133–141. doi: 10.1172/JCI7090

Figure 4.

Figure 4

(a) Representative Safranin O–stained section of incubated whole disc tissue. Wild-type whole disc tissue cocultured with peritoneal macrophages (mφ/wt) showed extensive reduction in staining compared with wild-type discs without macrophages (wt) or MMP-3–null whole disc tissue cocultured with macrophages (mφ/3–). ×250. (b) Quantitative analysis of wet weight loss in whole disc tissue from MMP-null organ cultures. Cocultures are indicated by the genotype of the mice donating macrophage/disc tissue. Wild-type discs were also treated with recombinant TNFα (rTNFα) and assayed for MMP-3 protein by Western analysis (inset) and wet weight (wt + rTNFα). Data were analyzed with the Mann-Whitney test and were presented as mean ± SD, n = 7 discs/group. * Difference in wet weight between before and 3 days after incubation is significant (P < 0.01). wt, wild-type; 3–, MMP-3 null; 7–, MMP-7 null.