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PGIS-overexpressing mice have reduced levels of F2-isoprostanes in the lung. PGIS OE+ mice and littermate control PGIS OE− mice were intranasally administered 0.08 U of bleomycin at day 0. The levels of F2-isoprostanes in the ground lung homogenate were determined by a gas chromatographic-negative-ion chemical ionization mass spectrometric assay. Data shown are the means ± SE, n = 5–6 mice, *P < 0.05 vs. PGIS OE−/bleomycin at the same time point.
