Fig. 8.

The PGI₂ analog cicaprost regulates Nqo 1 expression in Immortomice Type II epithelial cells in vitro. A: mRNA levels of PGI₂ IP receptor in the pulmonary Type II cells of Immortomice were determined by real-time PCR, and the PCR products were analyzed on an agarose gel. B: Type II cells were cultured and treated with cicaprost (2 μM) or vehicle (water). C: Type II cells were treated with indomethacin (2 μM) or vehicle solution at 4 h before treating with either cicaprost (2 μM) or vehicle (water). Bleomycin (0.1 U/ml) was added to the culture 30 min after cicaprost or vehicle treatment. The cells were harvested 4 h after cicaprost (B) or 4 h after bleomycin treatment (C), and the levels of Nqo 1 mRNA in the cells were determined by real-time PCR. Data shown are the means ± SE of 3 separate experiments. *P < 0.05 cicaprost (Cica)-treated cells compared with vehicle (Veh)-treated cells.