Fig. 4.

2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG) as an optical reporter of glucose consumption, from the micro to macro scale. A: 2-NBDG is avidly internalized and retained by HT-29 human colorectal cancer cells in vitro. B: 2-NBDG uptake can be imaged by spectral deconvolution fluorescence imaging in HT-29 subcutaneous tumors in mice. C: 2-NBDG localizes to adenomas generated by the intraperitoneal injection of the carcinogen azoxymethane in a mouse model of colorectal cancer, imaged by fluorescence colonoscopy. Adapted from Ref. 108.