(A) Growth of the indicated strains (WT, ppm1Δ, Pph21(L369A)/Pph22(L377A)) was monitored following switch from YPL to SL plus the indicated amino acids. Growth rates calculated as doublings per 8 hr are shown. Note that methionine alone improves growth in SL better than a mixture of other amino acids within this time period, via the methylation of PP2A.
(B) The indicated strains were grown in YPL or switched from YPL to SL. Mutants lacking the ability to methylate PP2A grow much more poorly under the more challenging conditions.