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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Toxicol Res (Camb). 2013 Feb 18;2(3):180–186. doi: 10.1039/C3TX20088D

Figure 3. DACT selectively depletes ER calcium stores in LβT2 cells.

Figure 3

LβT2 cells primed by GnRH exposure were exposed for 24 hrs to 300 μM DACT or vehicle control (DMSO) and examined for changes in intracellular Ca2+ following stimulation with either 10 μM thapsigargin (Tg) or 5 mM caffeine. (A,B) Tg-induced release of Ca2+ from ER stores was unaffected in LβT2 cells treated with DACT, relative to vehicle-treated control cells. (C,D) Caffeine-induced release of Ca2+ from ryanodine receptor-sensitive stores was reduced following treatment with 300 μM DACT. Data were combined from 3 independent experiments, averaging 500 - 600 cells total per experimental group. (*p<0.05)