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. 2011 Aug 19;301(5):L667–L674. doi: 10.1152/ajplung.00423.2010

Fig. 4.

Fig. 4.

Rho-kinase activity and RhoA activity. A: to determine the Rho-kinase activity, the level of phosphorylated myosin phosphatase target subunit 1 (MYPT1) on Thr696 (MYPT1Thr696) was measured by a Western blot analysis. The bands for phospho-MYPT1Thr696 were normalized to those of total MYPT1. The level of MYPT1 phosphorylation was upregulated in SM22-tet-BMPR2R899X mice. Treatment with fasudil apparently decreased the level of MYPT1 phosphorylation. B: to determine the RhoA activity, the level of GTP-RhoA was measured. The bands for GTP-RhoA were normalized to those of total RhoA. The level of GTP-RhoA was upregulated in SM22-tet-BMPR2R899X mice. Treatment with fasudil did not affect the level of GTP-RhoA. *P < 0.05. ns, Not significant.