Fig. 5.

Smad phosphorylation. A: no differences in the Smad1 phosphorylation levels were observed between the controls and SM22-tet-BMPR2^R899X mice. Treatment with fasudil did not affect the level of Smad1 phosphorylation. B: no differences in the Smad2 phosphorylation levels were observed between the controls and SM22-tet-BMPR2^R899X mice. Treatment with fasudil did not affect the level of Smad2 phosphorylation. The bands for phosho-Smad1^Ser463/465 and phospho-Smad2^Ser465/467 were normalized to those of β-actin.