Fig. 4.

Western blots showing prenylated vs. unprenylated RhoA levels under basal (0) and following 24-h pretreatment with different concentrations of simvastatin. Samples of tissue lysates (200 μg) were incubated with anti-farnesyl antibody. The prenylated proteins were immobilized and captured on protein G agarose beads. The proteins bound on beads were then denatured in sample buffer and separated by SDS-PAGE, followed by Western blotting using RhoA antibody. The supernatants containing unprenylated proteins were also analyzed. Data show a significant decrease in the levels of prenylated RhoA following simvastatin (*P < 0.05; n = 4).