Fig. 4.

Histograms showing relative expression of mRNA by real-time PCR of genes regulating adipose tissue mass and fatty acid metabolism expressed as fold change in PCA compared with SO control rats (solid bars) and the response to FSTN compared with PBS used a vehicle in the PCA rats (shaded bars). ACC, acyl-CoA carboxylase; Acyl-CoA Ox., acyl-CoA oxidase; Adiponectin Rec., adiponectin receptor; Fatty Acyl-CoA BP, fatty acyl-CoA binding protein; PPAR, peroxisome proliferator activated receptor; SREBP, sterol regulatory element binding protein. A: relative expression of myostatin was significantly elevated (P < 0.01) in WAT of PCA rat compared with SO rats. Relative expression of myostatin in WAT did not change (P > 0.1) in response to FSTN compared with vehicle (PBS) alone in the PCA rats. B: relative expression of mRNA by real-time PCR of adiponectin and its receptors in WAT of PCA rats. Adiponectin gene expression was significantly (P < 0.001) higher in WAT of PCA rats compared with SO control rats. Adiponectin receptor 1 expression was lower (P < 0.01), and that of adiponectin receptor 2 was higher (P < 0.01), in PCA compared with SO control rats. In response to FSTN, these alterations were reversed in the PCA rats compared with those administered vehicle (PBS) alone. C: relative expression of genes regulating fatty acid synthesis (fatty acid synthase, ACC, SREBP1, stearoyl-CoA desaturase) were significantly lower in PCA compared with SO control rats. In response to FSTN, a myostatin antagonist, these changes were reversed. D: the expression of genes regulating fatty acid oxidation (fatty acyl-CoA BP; acyl-CoA oxidase; PPAR-α) was significantly (P < 0.01) higher in the PCA compared with SO control rats. FSTN reversed these alterations in the PCA rats compared with those administered vehicle (PBS) alone. Values are means ± SD.