FIGURE 3.
MAG induces PTEN-dependent depression of PIP3 levels. A–C, pseudocolor time-lapse confocal images of PHAkt-GFP standard-corrected for growth cone thickness during basal conditions (A, n = 6; see also supplemental Movie S1) and with MAG treatment (150 ng/ml; starting at time = 0) either alone (B, n = 6; see also supplemental Movies S2–S4) or after down-regulating PTEN expression (C, PTEN-MO, n = 7; see also supplemental Movie S5). Time is in minutes. White is highest fluorescence intensity. Scale bar, 5 μm. D, quantification of change in fluorescence intensity as compared with the mean intensity prior to treatment (F/F0). Treatment groups are significantly different (p = 0.0029, repeated measures two-way ANOVA).